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how long to soak seeds in hydrogen peroxide

Hydrogen peroxide soak for seeds?

I have read on some other threads that many gardeners are having great luck with soaking older seeds in good ol’ hydrogen peroxide solution, that soaking seeds in hydrogen peroxide can help to get through the hard shell and increase germination? Has anyone here had any luck with hydrogen peroxide and germination?

Comments (6)

nandina

I have used this method for many, many years. Works well.

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backyardbrug

how long should brugmansia seed be soaked?

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jendudley

How old seeds and what type would benefit from this? Does anyone know the science behind this? Does it need to be warm hydroperox? How long a soak?

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echoingsprings

Well, i don’t know if it helps seedlings break through the seed coat, but I do know that soaking seeds in a 3% solution of hydrogen peroxide for 10-30 minutes, followed by at least 10 rinses in clean water will kill any pathogen growing on the outside of the seed. Germination appears increase after this sterilization because some seeds get attacked by pests as soon as germination begins and the embryo is destroyed before it even emerges from the soil. This is routinely done in plant research laboratories to prevent powdery mildew, mold etc. It also prevents damping off. You can also use a 3% solution of bleach, as long as the hypochlorite level is low: javex is an excellent brand. The cheaper bleaches are not as pure and won’t have the same effect. As for the hydrogen peroxide, I’d recommend using a food grade solution.

As for a ‘biological’ explaination, hydrogen peroxide is a plant signalling molecule (like ethylene) used by the plant to mediate pathogen and environmental stress responses. It also *MAY* help in root development. I’ve heard of some people actually watering or spraying plants with low doses of hydrogen peroxide to help plants overcome pathogen attack, or make them hardier if a pathogen make its way into the garden. Personally, I don’t like spraying my plants with too many chemicals, and if you do have a diseased plant, it is better to sacrifice it before it infects everything else. Plus, not much is known about hydrogen peroxide plant signalling yet, the hydrogen peroxide may stop pathogen attack, but what else is it telling your confused plant to do?

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albert_135 39.17°N 119.76°W 4695ft.

I took some honey locust Gleditsia (?) and scratched them with a file (My experience has been they may sometimes soak for a year without germination unless they go through some freeze/thaw cycles.) and I put some in a household peroxide soak and some in a bottled water soak. Both samples started to swell in about 12 hours. Those in the bottled water produced roots about 48-72 hours before those in the peroxide.

The unscrached controlls in water and in peroxide show no indication of doing anything as was expected.

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ljrmiller

It could have been coincidence, but I spritzed the pot with Echinocactus polycephalus seeds in it (and everything else in the flat) with 0.3% hydrogen peroxide (the drugstore stuff diluted 1/10) and the next day the seeds started germinating. Okay, so nothing else in the flat has germinated yet, so it probably IS coincidence.

I have read on some other threads that many gardeners are having great luck with soaking older seeds in good ol' hydrogen peroxide solution, that soaking seeds in hydrogen peroxide can help to get through the hard shell and increase germination? Has anyone here had any luck with hydrogen peroxide an…

How do you sterilize seeds with hydrogen peroxide?

Moreover, is hydrogen peroxide safe for seedlings?

Hydrogen peroxide is useful in variety of plants raised under hydroponic gardens, raised beds,or greenhouses. It seems to really support both good health and strong growth for plants. Hydrogen peroxide can also help with soil fungus. it aerates the soil, and it is anti-fungal.

Secondly, what does hydrogen peroxide do to seeds? Hydrogen peroxide (H 2O 2) pre-treatment increased germination percentage as well as the growth of pea seedlings in a concentration-dependent manner. The effects of H 2O 2 on germination were more prominent when seeds were incubated in darkness than under light conditions.

People also ask, how do you sterilize Microgreen seeds?

Soaking Seeds – eg: 9oz/16 oz cup water (sunflower)

  1. Use cold water (always pH balanced)
  2. Soak for 12-24 hrs typically.
  3. Refrigerate in warm weather.
  4. Put in colander drain, soak, rinse, mist seed thoroughly and keep damp for 24-36 hrs until they sprout in the colander. Treat 2-3 times daily.

How do you sterilize Arabidopsis seeds?

  1. Put your seeds in a 1.7 ml tube or a falcon tube (depends on the amount of your seeds). Add enough 70% EtOH.
  2. Pour out the EtOH. Add enough volume of 50% bleach.
  3. Discard the bleach.
  4. Wash seeds thoroughly with a large amount of sterile dH2O.
  5. Discard the water. Repeat step 3 for 3 more times.
  6. Your seeds are ready for use.

Hydrogen Peroxide: Add 5oz of 3% hydrogen peroxide (1oz if using 35% peroxide) and 1oz of vinegar to 33oz of room temperature water. Pour the solution over your seed and let it stand for 5 minutes. Make sure all of the seed is in contact with the solution.